Use the minus sign: `=A1-B1`
Subtract multiple cells: `=A1-B1-C1`
Subtract a number from a cell: `=A1-5`
Subtract cells across rows or columns by dragging the fill handle
Use parentheses to control order: `=(A1-B1)-C1`
Subtract dates: `=B1-A1`
Subtract times: `=B1-A1`
Use AutoSum dropdown and choose Sum, then subtract manually with `-`
Copy the formula to other cells to apply subtraction to more values