Find atoms that are tetrahedral and bonded to four single groups
Check whether the atom is attached to four different substituents
Exclude atoms with double or triple bonds
Exclude atoms with two identical substituents
Exclude atoms in symmetrical environments
Check ring systems for duplicate paths around the ring
Treat isotopes as different substituents if present
Consider lone pairs only when identifying stereogenic centers in special cases
Verify that swapping any two groups would create a different stereoisomer
Confirm that the center is not part of a plane of symmetry