Gently lift the kitten’s tail
Look at the shape and spacing of the openings
Female kittens usually have a vertical slit close to the anus
Male kittens usually have a round opening farther from the anus
Female kittens often look like an exclamation mark
Male kittens often look like a colon
Check for any visible testicles, usually after 6 to 8 weeks
Compare with a known male or female kitten if possible
If unsure, ask a veterinarian or breeder to confirm