Blot up as much urine as possible with clean paper towels or a dry cloth
Remove bedding and wash it separately
Mix equal parts white vinegar and water in a spray bottle
Lightly spray the stained area without soaking the mattress
Blot again with a clean cloth
Sprinkle baking soda over the damp area
Let the baking soda sit until completely dry
Vacuum up the dried baking soda
If odor remains, repeat the vinegar and baking soda process
For stubborn stains, apply an enzyme cleaner made for urine
Let the mattress air dry completely
Use a fan or open windows to speed drying
Place a waterproof mattress protector on the mattress after it is dry