Blot up any fresh urine immediately with paper towels or a clean cloth
Mix equal parts white vinegar and water
Lightly dampen a cloth with the solution and wipe the affected area
Dry the floor completely with clean towels
Sprinkle baking soda over the area and let it sit for several hours
Vacuum or sweep up the baking soda
Use an enzymatic cleaner made for pet urine on the affected spot
Follow the product instructions exactly
Wipe away any residue with a slightly damp cloth
Dry the wood thoroughly after cleaning
Repeat the enzymatic treatment if the smell remains
If urine has soaked into seams or under the finish, sand and refinish the area if needed
If the odor persists in subflooring, remove damaged boards and clean the subfloor
Avoid soaking wood floors with excess liquid
Test any cleaner on a hidden area first