Use a formula like `=A1-B1-C1`
Subtract a cell range with `=A1-SUM(B1:D1)`
Subtract one cell from multiple cells with `=A1-B1-C1-D1`
Use parentheses to control order, such as `=(A1-B1)-C1`
Copy the formula down with the fill handle for other rows
Use absolute references like `=$A$1-B1-C1` if needed
Subtract across rows with `=A1-A2-A3`
Subtract across columns with `=A1-B1-C1`